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KU Leuven (Leuven en Heverlee) KULAK KUL Brugge Nabestellingen KU Leuven IIW De Nayer Universiteit Antwerpen Universiteit Gent
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Home Platelet Proteomics

Platelet Proteomics

Principles, Analysis, and Applications

Angel Garcia-Alonso Yotis Senis
Hematology - general
Book
  • Platelet Proteomics - 9780470463376
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Content
The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics.  This chapter shows potential applications for platelet proteomics not yet carried out.  It includes examples of post-translational modifications (PTMs) analysis in platelets. The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), sub-proteome analyses (secretome/releasate, membrane proteins, organelles). Finally, the last section links the platelet transcriptome and application to disease.  This section is highly relevant and includes chapters on proteomics, transcriptomics, functional genomics, systems biology, and their applications to platelet-related diseases. CONTENTS Preface by Ángel and García ad Yotis A. Senis. Foreward by Steve P. Watson. Part I. General overview: platelets, sample preparation and mass spectrometry-based proteomics. 1.Platelets and their role in thrombotic and cardiovascular disease: the impact of proteomic analysis (Ronald Stanley and Jon Gibbons). 2. Mass spectrometry-based proteomics: General overview, and PTMs analysis in the context of platelet research (Julia Burkhart and Albert Sickmann). 3. Sample preparation variables in platelet proteomics for biomarker research (Maria Zellner and Rudolf Oehler). Part II. Analysis of the platelet proteome: Global approaches and sub-proteomes. 4. Two-dimensional gel electrophoresis: Basic principles and application to platelet signaling studies (Ángel García). 5. The platelet membrane proteome (Yotis A. Senis). 6. Organellar proteomics and the analysis of the platelet releasate (James McRedmond). 7. The platelet microparticle proteome (David Smalley). 8. N-terminal COFRADIC analysis of the human platelet proteome (Kris Gevaert et al.). Part III. Integrated –omics and application to disease. 9. SAGE to study the platelet and megakaryocyte transcriptome (Mike Tomlimson). 10. The application of microarray analysis and its integration with proteomics to study platelet-associated disorders (Wadie Bahou and Dimitri Gnatenko). 11. Platelet Functional Genomics (Isabelle I. Salles et al.) 12. The Systems Biology to study platelet-related bleeding disorders (Jan Willem Akkerman and Bernard de Bono). 13. Platelet Proteomics in Transfusion Medicine (Andreas Greinacher and Thomas Thiele). 14. Cardiovascular proteomics (Fernando Vivanco et al.).
Specifications
Publisher
John Wiley and Sons Ltd
Publication date
May 10, 2011
Pages
352
ISBN
9780470463376
Format
Hardback

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