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Molecular Biology by Robert Weaver is designed for an introductory course in molecular biology. Molecular Biology 5/e focuses on the fundamental concepts of molecular biology emphasizing experimentation. In particular author, Rob Weaver, focuses on the study of genes and their activities at the molecular level. Through the combination of excellent illustrations and clear, succinct writing students are presented fundamental molecular biology concepts. Key features # Guide to Experimental Techniques -- A summary guide to more than 75 experimental techniques, referenced to the pages on which the techniques are discussed, is found in the front matter. # Would your students benefit from an overview of the extensive list of experimental techniques used in molecular biology? # Information on Affinity Chromatography --Chapter 5 (Molecular Tools for Studying Genes and Gene Activity) now includes information on Affinity Chromatography, one of the most powerful separation techniques, and also touches on Forensic Uses of DNA Fingerprinting and DNA Typing. # Material from Original Publications --Significant data and autoradiographs from original publications are included to support the narrative and to help students analyze data. # Analytical Questions -- As part of the End-Of-Chapter material, these questions encourage students to use outside tools to further their understanding of that particular chapter's concepts. (Answers will be provided on the new Fourth Edition website.) # New material on Eukaryotic RNA -- Chapter 10 (Eukaryotic RNA Polymerases and Their Promotors) includes lots of new material, inlcuding Three Dimensional Structure of RNA Polymermase II in the Post-Translocation State and info on Rpbl's interaction with the DNA-RNA hybrid. # New Information on Structure and Function of eIF3 -- Chapter 17 (The Mechanism of Translation I:Initiation) includes new information on the Structure and Function of eIF3 as well as Shifts in mRNA Secondary Structure. # New Informaton on the X Chromosome and more! -- Chapter 24 (Genomics, Proteomics, and Bioinformatics) includes new information on the X Chromosome and its role in sex-linked diseases. Also discussed in this chapter is the "Barcode of Life", a section connecting DNA to how taxonomists now classify organisms. A few other new topics touched upon is Whole Chromosome Transcriptional Mapping, Genomic Functional Profiling, Tissue-Specific Functional Profiling, and RNAi Analysis Table of contents 1 Introduction 1 A Brief History 2 The Molecular Nature of Genes 3 An Introduction to Gene Function 2 Methods of Molecular Biology 4 Molecular Cloning Methods 5 Molecular Tools for Studying Genes and Gene Activity 3 Transcription in Prokaryotes 6 The Mechanism of Transcription in Prokaryotes 7 Operons: Fine Control of Prokaryotic Transcription 8 Major Shifts in Prokaryotic Transcription 9 DNA-Protein Interactions in Prokaryotes 4 Transcription in Eukaryotes 10 Eukaryotic RNA Polymerases and Their Promoters 11 General Transcription Factors in Eukaryotes 12 Transcription Activators in Eukaryotes 13 Chromatin Structure and Its Effects on Transcription 5 Posttranscriptional Events 14 Messenger RNA Processing I: Splicing 15 Messenger RNA Processing II: Capping and Polyadenylation 16 Other RNA Processing Events 6 Translation 17 The Mechanism of Translation I: Initiation 18 The Mechanism of Translation II: Elongation and Termination 19 Ribosomes and Transfer RNA 7 DNA Replication, Recombination, and Transposition 20 DNA Replication I: Basic Mechanism and Enzymology 21 DNA Replication II: Detailed Mechanism 22 Homologous Recombination 23 Transposition 8 Genomes 24 Genomics and Proteomics
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