Table of Contents:
Section 1: Introduction to Molecular Testing
1. Basic Concepts in Molecular Pathology - Introduction to Molecular Testing in Human Disease
William B. Coleman and Gregory J. Tsongalis
2. Laboratory Approaches in Molecular Pathology - Polymerase Chain Reaction and Other Amplification Techniques
William B. Coleman and Gregory J. Tsongalis
3. Laboratory Approaches in Molecular Pathology - Next Generation Sequencing
Nirali M. Patel and Daniel L. Duncan
4. Laboratory Approaches in Molecular Pathology - Automation in the Molecular Pathology Laboratory
Gregory J. Tsongalis and Scot Turner
Section 2:Molecular Testing in Infectious Disease
5. Molecular Testing for Human Immunodeficiency Virus (HIV)
Bruno Pozzetto
6. Molecular Testing in Hepatitis Virus-related Disease (HBV, HCV)
Tomasz I. Michalak
7. Molecular Testing for Human Papilloma Viruses
Katie M. Bennett
8. Molecular Testing for Herpes Viruses
Benjamin A. Pinsky
9. Molecular Testing for Parvoviruses
Giorgio Gallinella
10. Molecular Testing for Polyomaviruses
Belinda Yen-Lieberman and Gary W. Procop
11. Molecular Testing for Respiratory Viruses
Kathleen A. Stellrecht
12. Molecular Testing for Diseases Associated with Bacterial Infection
Melissa Miller
13. Infectious Agents Associated with Sexually-transmitted Diseases
Bruno Pozzetto
14. Infectious Agents Associated with Nosocomial Infection (MRSA, C. difficile)
Rodney Climaco Arcenas
15. Molecular Testing in Emerging Infectious Diseases
David H. Walker, Jianli Dong and Nahed Ismail
Section 3: Molecular Testing in Genetic Disease
16. Non-invasive Prenatal Molecular Testing
W. Edward Highsmith Jr.
17. Molecular Testing in Cardiovascular Disease
Heather McLaughlin
18. Molecular Testing for Coagulopathies
Peter L. Perrotta and Matthew Smolkin
19. Molecular Testing in Cystic Fibrosis
Hunter Best
20. Molecular Testing of Hemochromatosis
Pierre Brissot
Section 4: Molecular Testing in Oncology
21. Molecular Testing in Breast Cancer
Kimberly H. Allison
22. Molecular Testing in Prostate Cancer
Scott A. Tomlins
23. Molecular Testing in Lung Cancer
Ming-Sound Tsao and Carolyn J. Shiau
24. Molecular Testing in Colorectal Cancer
Chanjuan Shi and Jessica Suzanne Thomas
25. Molecular Testing in Melanoma
Gregory J. Tsongalis and Amin Hedayat
26. Molecular Testing for Glioblastoma
Dimitri Trembath
27. Molecular Testing in Pancreatic Cancer
Massimo Raimondo
28. Molecular Testing in Gynecologic Cancer
Sarah E. Kerr
29. Molecular Testing in Renal Cancers
Harry A. Drabkin and Kathryn Marie Willoughby
30. Molecular Testing in Thyroid Cancers
Yuri E. Nikiforov
31. Molecular Testing for Pediatric Cancers
Kathleen W. Rao and Kristy Crooks
Section 5: Molecular Testing in Hematopathology
32. Molecular Testing in Chronic Myelogenous Leukemia
Noah A. Brown and Bryan L. Betz
33. Molecular Testing in Acute Myelogenous Leukemia
Bryan L. Betz and Amir Behdad
34. Molecular Testing in Myeloproliferative Neoplasms
Bryan L. Betz and Larissa V. Furtado
35. Molecular Testing in the Assessment of Bone Marrow Transplant Engraftment
Jessica Booker
Section 6: Molecular Testing in Personalized Medicine
36. Personalized Medicine in Cardiovascular Disease (CYP2C19)
Munir Pirmohamed and Richard Turner
37. Personalized Medicine for Coagulopathies (CYP2C9, VKORC1)
Jawed Fareed
38. Personalized Medicine for Hepatitis C
Andrea Ferreira-Gonzalez
39. Personalized Medicine in Cancer Treatment
Victoria M. Pratt and Stuart Scott
Section 7: The Future of Molecular Testing
40. Mass Spectroscopy in the Molecular Pathology Laboratory
Richard M. Caprioli
41. Whole-genome Sequencing in the Molecular Pathology Laboratory
Jonathan S. Berg
Case Studies Q&ASection 1: Introduction to Molecular Testing
1. Basic Concepts in Molecular Pathology - Introduction to Molecular Testing in Human Disease
William B. Coleman and Gregory J. Tsongalis
2. Laboratory Approaches in Molecular Pathology - Polymerase Chain Reaction and Other Amplification Techniques
William B. Coleman and Gregory J. Tsongalis
3. Laboratory Approaches in Molecular Pathology - Next Generation Sequencing
Nirali M. Patel and Daniel L. Duncan
4. Laboratory Approaches in Molecular Pathology - Automation in the Molecular Pathology Laboratory
Gregory J. Tsongalis and Scot Turner
Section 2:Molecular Testing in Infectious Disease
5. Molecular Testing for Human Immunodeficiency Virus (HIV)
Bruno Pozzetto
6. Molecular Testing in Hepatitis Virus-related Disease (HBV, HCV)
Tomasz I. Michalak
7. Molecular Testing for Human Papilloma Viruses
Katie M. Bennett
8. Molecular Testing for Herpes Viruses
Benjamin A. Pinsky
9. Molecular Testing for Parvoviruses
Giorgio Gallinella
10. Molecular Testing for Polyomaviruses
Belinda Yen-Lieberman and Gary W. Procop
11. Molecular Testing for Respiratory Viruses
Kathleen A. Stellrecht
12. Molecular Testing for Diseases Associated with Bacterial Infection
Melissa Miller
13. Infectious Agents Associated with Sexually-transmitted Diseases
Bruno Pozzetto
14. Infectious Agents Associated with Nosocomial Infection (MRSA, C. difficile)
Rodney Climaco Arcenas
15. Molecular Testing in Emerging Infectious Diseases
David H. Walker, Jianli Dong and Nahed Ismail
Section 3: Molecular Testing in Genetic Disease
16. Non-invasive Prenatal Molecular Testing
W. Edward Highsmith Jr.
17. Molecular Testing in Cardiovascular Disease
Heather McLaughlin
18. Molecular Testing for Coagulopathies
Peter L. Perrotta and Matthew Smolkin
19. Molecular Testing in Cystic Fibrosis
Hunter Best
20. Molecular Testing of Hemochromatosis
Pierre Brissot
Section 4: Molecular Testing in Oncology
21. Molecular Testing in Breast Cancer
Kimberly H. Allison
22. Molecular Testing in Prostate Cancer
Scott A. Tomlins
23. Molecular Testing in Lung Cancer
Ming-Sound Tsao and Carolyn J. Shiau
24. Molecular Testing in Colorectal Cancer
Chanjuan Shi and Jessica Suzanne Thomas
25. Molecular Testing in Melanoma
Gregory J. Tsongalis and Amin Hedayat
26. Molecular Testing for Glioblastoma
Dimitri Trembath
27. Molecular Testing in Pancreatic Cancer
Massimo Raimondo
28. Molecular Testing in Gynecologic Cancer
Sarah E. Kerr
29. Molecular Testing in Renal Cancers
Harry A. Drabkin and Kathryn Marie Willoughby
30. Molecular Testing in Thyroid Cancers
Yuri E. Nikiforov
31. Molecular Testing for Pediatric Cancers
Kathleen W. Rao and Kristy Crooks
Section 5: Molecular Testing in Hematopathology
32. Molecular Testing in Chronic Myelogenous Leukemia
Noah A. Brown and Bryan L. Betz
33. Molecular Testing in Acute Myelogenous Leukemia
Bryan L. Betz and Amir Behdad
34. Molecular Testing in Myeloproliferative Neoplasms
Bryan L. Betz and Larissa V. Furtado
35. Molecular Testing in the Assessment of Bone Marrow Transplant Engraftment
Jessica Booker
Section 6: Molecular Testing in Personalized Medicine
36. Personalized Medicine in Cardiovascular Disease (CYP2C19)
Munir Pirmohamed and Richard Turner
37. Personalized Medicine for Coagulopathies (CYP2C9, VKORC1)
Jawed Fareed
38. Personalized Medicine for Hepatitis C
Andrea Ferreira-Gonzalez
39. Personalized Medicine in Cancer Treatment
Victoria M. Pratt and Stuart Scott
Section 7: The Future of Molecular Testing
40. Mass Spectroscopy in the Molecular Pathology Laboratory
Richard M. Caprioli
41. Whole-genome Sequencing in the Molecular Pathology Laboratory
Jonathan S. Berg
Case Studies Q&A
William B. Coleman
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